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BGB-02 Geomicrobiology: Low-temperature alteration, mineralization, and microbial interactions
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Distribution of microorganism in the unsaturated zone and groundwater beneath the wastewater irrigation area
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Cuiyun Zhang, Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences (China)
Miying Yin, Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences (China)
Zhenghong Li, Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences (China)
Linna Ma, Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences (China)
Sheng Zhang, Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences (China)
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21 groups of samples collected from 5m deep the unsaturated zone and groundwater in the wastewater irrigation area near Shijiazhuang, China were used for analysis of microorganism, including total heterotrophic aerobes, total heterotrophic anaerobes, denitrifiers, iron-oxidizing bacteria, iron-reducing bacteria, sulfate-reducing bacteria and methanogenic bacteria by dilution plating and a three-tube MPN methods. The results of investigation showed that total heterotrophic aerobes, total heterotrophic anaerobes, denitrifiers, iron-oxidizing bacteria, iron-reducing bacteria were all distributed in the unsaturated zone and groundwater, and their abundance ranged from n102 cells/(g or mL) to n107(cells/g or mL). However, sulfate-reducing bacteria and methanogenic bacteria were only distributed in the upper of the unsaturated zone (<100cm). Geological texture, moisture content and organic carbon had significant influence on microbial distribution. The results of analysis were the basis of assessing biological natural attenuation of contaminants in the unsaturated zone and groundwater.
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